Matthew Azizi

About Matthew Azizi

Adaptable analytical scientist with 4+ years of industrial experience in CMC development across various pharmaceutical clinical stages for both small and large molecule entities. Strong troubleshooting and problem-solving skills, experienced in developing and optimizing chromatographic methods for diverse R&D projects across various modalities, including small molecules, peptides, mAbs, and ADCs. Proficient in proteomics and mass spectrometry characterization of proteins and peptides. Skilled in executing all aspects of projects, from designing experiments and developing ideas to troubleshooting, data interpretation and review in both R&D and GMP settings, report writing, presentation, and completion within a given timeframe.Analytical Research & Development (Postdoc), Merck & Co. Characterization of drug substances and drug products by using orthogonal 2DLC-MS approaches in a cross-functional collaboration with multiple ARD groups. Structure determination of unknown epimer impurity which coelutes with API in clinical batch under LCAP method conditions, by developing new achiral × chiral 2DLC-MS/MS procedure. Developing 2D-SEC×RP-LC/MS and 2D-SEC×SEC-LC/MS methods for detection of Host Cell Proteins (HCPs) and unknown HMW species in PEGylated DS at the intact level. Studying charged variants of the light stressed mAbs at different forced degradation conditions by implementing IEX×RP 2DLC-MS. Designing extremely high resolution 2D RP×RP separation for characterization of HCPs by peptide mapping. Analytical Research & Development (Intern), Merck & Co. Optimized searching parameters of LC-MS based proteomics analysis for identification of HCPs. Facilitated data analysis of HCP detection by using LabKey as a data repository platform. Studied the effect of molecular features, up-steam and down-stream conditions on HCP purification through different purification steps, such as protein A chromatography, AEX, CEX/HIC, and ultra-filtration. Tracked HCPs that involve drug degradation, such as lipases and esterases, in different purification steps. Technical Skills, Instrumentation, and Softwares: Two-dimensional Liquid Chromatography (2DLC) Tandem Mass Spectrometry (MS/MS) Proteomics Gas Chromatography (GC) Method Development Spectroscopy (UV-Vis, FT-IR) Microbial Culture Softwares: Empower, Proteome Discoverer, MaxQuant, MassLynx (Waters), X-Caliber (Thermo), OpenLab (Agilent), LabKey, LabSolutions (Shimadzu), ChemStation (Agilent), MSDIAL, DryLabYou can send me an email at a••••••••@gmail.com

Experience

1. Sr Scientist, Analytical Development & Operations

   Gilead Sciences

   Jan 2025 — Present · Foster City, CA, US

Education

• Shiraz University

  Master's Degree, Chemical Engineering

  2011 — 2014

• Shiraz University

  Bachelor of Science (B.Sc.), Chemical Engineering

  2006 — 2010

• The University of Texas at Arlington

  Doctor of Philosophy (PhD), Analytical Chemistry

  2015 — 2020