Manjunath Rao A

Research Associate @Sarsuagenterprises

Bengaluru, IN

Protein Biochemistry & Purification:Routinely performed SDS-PAGE, Western Blotting, and purification protocols, successfully increasing target protein yield by 5% through optimization of extraction and column chromatography methods.Accurately quantified protein concentrations using the Bradford assay to standardize reagents for subsequent assays and product formulation.Immunology & Assay Management:Managed a significant weekly assay workload, running 5-6 ELISA plates for quantitative measurement of antibody titer, protein-ligand binding, and biomarker detection.Executed comprehensive mice immunization protocols to generate high-quality polyclonal antibodies essential for ongoing research and product development.Cell Culture & Technical Operations:Maintained and managed 5-7 mammalian cell culture flasks weekly under strict aseptic conditions to provide biological material for functional assays and host-cell studies.Contributed technical expertise to unique product research, including the systematic study and characterization of venom components and their potential applications.Methodology & Quality Control:Ensured data integrity and experimental reproducibility by strictly adhering to and documenting Standard Operating Procedures (SOPs) across all purification and assay platforms.