Madhu Sudana Chikka

Process Manager @Rentschler Biopharma

Milford, MA, US
EMAILS
m••••••••@rentschler-biopharma.com
MOBILE NUMBERS
+91 *********19

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WORK HISTORY

Jun 2022 — Present

Process Manager @Rentschler Biopharma

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Milford, MA, US

EDUCATION

N/A

Andhra Loyola College, Vijayawada

Bachelor of Science (B.Sc.)

2003 — 2009

Purdue University

Doctor of Philosophy (Ph.D.), Biology/Biological Sciences, General

2000 — 2002

University of Hyderabad

Masters, Plant Sciences

SKILLS

Protein ExpressionMolecular CloningConfocal MicroscopyWestern BlottingQpcrTissue CultureCellMolecular BiologyRt-PcrCell CultureCell BiologyDnaFluorescence MicroscopyImmunohistochemistryTransfectionDna SequencingProtein ChemistryImmunoprecipitationElisaBiochemistryPcrGeneticsMicroscopyRna IsolationImmunofluorescenceSds-PageAnimal ModelsIn VitroPurificationSequencingProtein PurificationFluorescenceSite-Directed MutagenesisModel Systems: Caenorhabditis Elegans, Drosophila Melanogaster, Mus MusculusLife SciencesPolymerase Chain Reaction (Pcr)Reverse Transcription Polymerase Chain Reaction (Rt-Pcr)

ABOUT MADHU SUDANA CHIKKA

3+ years of industry experience as a Scientist and as a Team Lead in cGMP Biologics producing CRO & CMO set up.2+ years of industry experience as a Scientific Applications Specialist in the field of advanced molecular biology and genomics.5+ years of postdoctoral experience in Cell and Molecular Biology in academic set up.Techniques:Biologics: small-scale (CR & D) and large-scale (Manufacturing), cGMP processes: Upstream and Downstream process development, Chromatography (Ion Exchange, Affinity, Size Exclusion), Sterile Technique, Mammalian cell culture, Next Generation Sequencing, Genotyping assays, Quantitative RT-PCR, Digital droplet PCR, Cloning, PCR, Sanger Sequencing, RNAi technology, ASO (Antisense Oligonucleotides), CRISPR, Western blotting, HPLC, Capillary Electrophoresis, In vitro Cell-Based Assays, Quantitative Assays (luminescence, colorimetric), Chromatin Immunoprecipitation, Flow Cytometry, Microscopy (confocal and electron microscopy).

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