Maddalena Palmieri
Supervisor, Qc Biochemistry @Thermo Fisher Scientific
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WORK HISTORY
Supervisor, Qc Biochemistry @Thermo Fisher Scientific
Ferentino, IT
EDUCATION
DFM S.c.a.r.l. - IBB-CNR , Naples
Training Course for qualified staff oriented to the biotechnology for pharmaceutical chemistry
Second University of Naples
Bachelor Degree in Biotechnologies
Second University of Naples
Master's degree
Second University of Naples
Doctor of Philosophy (Ph.D.)
SKILLS
ABOUT MADDALENA PALMIERI
Principal subjects/occupational skills covered as PDS AD & GMP Scientist and Scientist II - Development and validation of Analytical Methods at lab scale to support formulation development, stability testing and quality control testing of new pharmaceutical products ensuring delivery in accordance with project timescales and deadlines- Development and validation of Analytical Methods for testing pharmaceutical products at manufacturing scale in compliance to Good Manufacturing Practice (GMP) requirements- Development and validation of test methods for use during cleaning validation- SOP, Protocols & Reports writing, editing and reviewing- Supervising of analytical development activities and analytical development team. Principal subjects/occupational skills covered as Researcher and Post Doc - Assays for peptides stability in drug development - In vitro peptides serum (Human and Murine) stability determined by RP-HPLC and HPLC - microRNA formulation development to improve in vivo microRNA delivery for cancer therapy HPLC and RP-HPLC (Agilent 1260 Infinity Binary System equipped with DAD detector and Autosampler- OpenLab CDS Software); UV-Vis; Electrophoresis (SDS-PAGE)-Protein folding mechanisms characterization using advanced experimental spectroscopic techniques as nuclear magnetic resonance (NMR), circular dichroism (CD) and differential scanning calorimetry (DSC) NMR (500 MHz Varian Unity Inova Spectrometer equipped with 4 channels (1H, 13C, 15N, 31P) and Pulsed Field Gradient – VnmrJ experiments Software), CD (JASCO J815 CD Spectropolarimeter equipped with Peltier Temperature control – Spectra Manager Software)-Over-expression, purification and characterization (AKTA-purifier; UPLC/HPLC) of unlabeled, single-labeled (15N) and double-labeled (15N and 13C) proteins used for the NMR and other spectroscopic techniques Purification by FPLC using Unicorn Control Software for chromatographic systems
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