Luke Daniel Ofria

About Luke Daniel Ofria

Current Graduate Student Researcher at the Johns Hopkins University School of Medicine and Whiting School of Engineering.Former Research Associate at CreyonBio, Inc, a preclinical pharmaceutical startup leveraging machine learning models and AI to design oligonucleotide-based medicines for cost-effective treatments of rare genetic diseases. A seasoned researcher with nearly three years of experience in the biotechnology industry.My relevant skills:Next Generation Sequencing – development and optimization of customized and multiplexed library preparation for allele-specific sequencing, medium-scale transcriptomics, and large scale DGE analysis; high-throughput multiplexed library preparations; experience with ONT Nanopore, Illumina MiniSeq, and ElementBio Aviti platformsOligonucleotide Based Medicine (OBM) Drug Candidate Screening – screening of potential drugs via NGS assays for OBM activity (allele specific knockdown and splice shifting) and off-target effectsAutomation – experience with Hamilton NGS Star, Echo Acoustic Liquid Handler, Integra Viaflo and Opentrons robotic platformsBioinformatics – proficient in Python, Unix, Snakemake, MATLAB and R for the creation and customization of NGS pipelines; Geneious Prime, JBrowseCell Culture & Gene Editing – human, plant and bacterial cell culture, FACS, UV mutagenesis, forward genetic screening, CRSPIR Cas9 targeted gene editing, RNA extraction

Experience

1. Graduate Research Assistant

   Johns Hopkins Whiting School Of Engineering

   Feb 2025 — Present · Baltimore, MD, US

   Kathuria LabSmith Building

Education

• The Johns Hopkins University

  Master of Science - MS, Bioinformatics

• Eastlake High School

  High School Diploma

  2013 — 2017

• University of California, Berkeley

  Bachelor of Science - BS, Bioengineering

  2017 — 2021