Lorillee Tallorin

Senior Research Scientist at Vertex Pharmaceuticals

Role
Senior Scientist at Vertex Pharmaceuticals
Location
Boston, MA, US
LinkedIn followers
500 followers
Research & DevelopmentView LinkedIn profile

About Lorillee Tallorin

Ph.D. level scientist -collaborative and motivated chemical biologist/enzymologist with expertise performing small-molecule discovery screens to characterize molecular mechanisms of potential biologic therapeutics. Experience with analysis of protein structure and function, while working with complex biomolecules for characterizing protein and RNA interactions, and understanding enzymatic activity to characterize mechanism of action. Areas of Expertise:• Drug Discovery • Mechanism-of-Action Studies• Natural products • Molecular, Cellular, and Biochemical AssaysSpecialties: Assay Development, biophysical protein characterization, protein-protein interaction, RNA assays, small-molecule inhibitor discovery.Laboratory Skills:Biochemistry & Molecular Biology | Bacterial protein expression • microplate-based enzyme assays • enzymatic absorbance/luminescence/ FRET/alphaLISA/fluorescence-labeling/mass spec/HPLC assays • alpha-screen binding assay • Isothermal Titration Calorimetry (ITC) • Bio-layer interferometry (BLI) • mammalian cell culturing • immunofluorescence microscopy • immunoprecipitation/affinity-pull down • protein purification-size-exclusion/ion-exchange (ÄKTA system) • protein and peptide fluorescent labeling • molecular cloning & PCR • Western blot • liposome mediated transfection• in vitro transcription and translation • RT PCR Chemical Biology | LC-MS • HPLC • FT-IR spectroscopy • UV-Vis Spectroscopy • bottom-up proteomics • peptide synthesis (SPOT array-Intavis) • small molecule probe design for protein • protein interactionSoftware & Computation | GraphPad Prism • ChemDraw • PyMOL • SnapGene/Geneious • Adobe Suite Agilent Mass Hunter LC-MS • ImageJ/Fiji

Experience

  1. Senior Scientist

    Vertex Pharmaceuticals

    Feb 2023 — Present · Boston, MA, US

Education

  • California State University, Los Angeles

    Master of Science (M.S.), Chemistry

    2008 — 2010

  • UCLA

    Bachelor's Degree, Chemistry

    2005 — 2007

  • Palomar College

    Associate's Degree, Chemistry

    2003 — 2005

  • Harvard Medical School

    Postdoctoral Fellow, Chemical Biology & Virology

    2016 — 2019

  • UC San Diego

    Doctor of Philosophy (PhD), Chemical Biology/Biochemistry

    2010 — 2016

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Lorillee Tallorin — Senior Scientist at Vertex Pharmaceuticals in Boston, MA, US | Unifers