Kranthi Kumar Paladugu

About Kranthi Kumar Paladugu

Form and function is an interaction between Genome and environment. The molecular genetic changes and environmental interaction leads into a disease development. Identification of those molecular genetic diagnostic changes early could potentially pave the way to develop or manage a suitable treatment or management options. Imprinting and Genomic alterations could serve as useful diagnostic markers. These genetic markers and or altered genetic states could be used to identification and predicting a potential disease outcome.DNA Methylation playing a role in stabilization / protection of genome and amplification / deamplification changes in Aneuploids and Alien addition lines.Loss H. vulgare genetic material, NOR region shown first time. Cloned, characterized, sequenced and comparative phylogeny analysis done on Industrially important Laccase genes from Ganoderma lucidum. Replication slippage assay, dual antibiotic marker construct. Loss/Gain function studies and new electroporation transformation method developed for Chlamydomonas. AFLP technique used to identify Vernalization mediated DNA methylation changes in Arabidopsis.Trained & supervision MS and Ph.D students in Molecular biology techniques.Genetic diagnosis, consultation, counseling and risk assessment, R&D projects, Green house Lab and equipment Maintenance.Developed Anther culture Haploids, Mutagenesis, Variegation plants, Doubled haploids, Plant Tissue culture, Cell Culture. LEAN Manufacturing process, VAM. GLP, GMP, GCP, SOPs, QCs.Specialties:• Extensive experience in molecular biological techniques.•RFLP, AFLP, DNA Methylation. •Various PCR Techniques. • Vector construction. •Labelling & Detection. • HPLC.• Protein Purification and Charecterization• Plant Tissue culture, Embryo culture, Loss/ gain of Function.• Barley- self and cross pollination in the field and greenhouse environment.• Vernalization of Arabidopsis and Barley.• Mutagenesis •Bio-hazardous Material handling.

Experience

1. Business Development Manager

   Spls

   Sep 2013 — Present

Education

• Oregon Bioscience Association

  Educational Training Programs, Medical devices QA and FDA Regulation

  2014 — 2014

• University of Hyderabad

  Ph.D, Plant Molecular Genetics (DNA Methylation and RFLP variation, Chromosomal Elimination)

  1989 — 1998

• University of Hyderabad

  MscLife Sciences, Life Sciences

  1986 — 1988

Skills

• Hplc
• Rflp
• Dna
• Bioinformatics
• Lifesciences
• Purification
• Protein Expression
• Rna Isolation
• Virus Screening
• Molecular Cloning
• Science
• Biochemistry
• Genotyping
• In Vitro
• Quality Control
• Drug Discovery
• Laboratory Maintainance
• Genetics
• Research
• Gel Electrophoresis
• Genetic Diagnostics
• Dna Extraction
• Western Blotting
• Plant Tissue Culture
• Pcr
• Tissue Culture
• Rt-Pcr
• Insertional Inactivation
• Saftey Committees
• Molecular Biology
• Micro Biology
• Elisa
• Fda Regulations Compliance
• Genomics
• Qpcr
• Cell
• Vector Construction
• Microbiology
• Mutagenesis
• Assay Development