Khushboo Malhotra

Microbiology Technician @Isopharm

Sheffield, GB

MICROBIOLOGY LABORATORY DUTIES• Work according to ISO 17025 standards and follow the procedures set out with the Quality Management System (QMS).• Water Quality Testing as per UKAS standards mainly using Membrane filtration. Develop strategic ways to solve water quality issues, read culture plates, and identify specific hospital pathogens such as Pseudomonas aeruginosa and Mycobacterium spp. • Perform various microbial identification confirmation tests such as Oxidase, catalase, Gram staining, and Carbol-fuchsin staining. • Conduct weekly and annual water testing from Endoscope washer-disinfectors (EWDs) and drying cabinets, mainly using Membrane filtration for total viable count (TVC) and detection of other hospital pathogens. • Obtain a variety of air and surface samples from different sites at the laboratory to perform environmental monitoring and enumerate the microbial growth from the environmental monitoring plates. • Perform DNA extraction and qPCR for the identification and quantification of mycobacteria in water samples. • Detect and quantify bacterial endotoxins using a Kinetic Chromogenic LAL assay.• Expert in endoscopy water testing using sterile water testing medical devices called Surrogates. Participate in External Quality Assessment (EQA) schemes by the UK Health Security Agency.• Perform audits on analytical methods and quality systems.• Efficiently use Laboratory Information Management System (LIMS). CHEMISTRY LABORATORY DUTIES• Automatic colourimetric determination of Phosphate, Silica, Total Hardness, Chloride, Nitrate, Ammonia and Sulphate by Gallery Plus discrete analyser.• Determination of pH and electrical conductivity by Skalar Robot.

The University of Sheffield

Master's degree, Molecular biology and biotechnology, First division with merit

Amity University

Bachelor of Science - BS, Microbiology, General, First division with merit