Klara Bojanovic MacHado

About Klara Bojanovic MacHado

I am a highly-motivated and innovative scientist with multidisciplinary skills of molecular biology, metabolic engineering, bioinformatics, product development, and business. I am adaptable and strive towards excellence. I thrive in cross-disciplinary teams with focus on high performance. I have experience in strain construction, non-coding RNA, RNAi, biochemistry, library prep & next-gen sequencing product development, and assay development and optimisation.Skills set:1. Scientific skills- molecular biology and metabolic engineering: nucleic acid isolation and manipulation (purification, amplification, labeling, hybridization), USER cloning, homologous recombination, restrictions, ligations, primer & vector design, PCR (conventional PCR, overlap PCR, touch-down PCR, RT/q-PCR, colony PCR), gel electrophoresis, genotyping- RNA techniques: RNA isolation, analysis, and manipulation, Northern blot, RNAi- protein techniques: SDS-PAGE, protein purification and quantification, Western blot, co-immunoprecipitation- sequencing: cDNA library preparation (Illumina), next-generation sequencing (RNA-seq, dRNA-seq, RIP-seq), Sanger sequencing- bioinformatic skills: analysis and drawing conclusions from large datasets, RNA-seq data analysis, statistics, basic programming in R and Unix, NGS visualization (IGV)- microbiology: high-throughput screening assays, reporter assays, strain characterization, growing cultures (bacteria, yeast, fungi), collecting samples, microscopy, UV-VIS2. Extarordinary communication skills including writing and oral3. Teamwork and leadershp skills4. Product Development Process: SOP, AmpliSeq for Illumina5. Business skills: Business plan development6. Software/Design skills: Microsoft Office, Adobe Illustrator and Photoshop, CLC Workbench, R, IGV, JMP, SAV

Experience

1. Senior Research Scientist at Amazon

   Amazon

   Oct 2021 — Present · Seattle, WA, US

Education

• DTU - Technical University of Denmark

  Doctor of Philosophy (PhD), Biotechnology

  2013 — 2016

• Faculty of Biotechnology, University of Ljubljana

  Bachelor of Science (BS), Microbiology

  2007 — 2010

• Faculty of Biotechnology, University of Ljubljana

  Master of Science (MS), Microbiology, General

  2010 — 2013

Skills

• Qpcr
• Gel Electrophoresis
• Bacterial Genetics
• Sds-Page
• Polymerase Chain Reaction (Pcr)
• Yeast
• Rt-Pcr
• Dna Extraction
• Rnaseq
• Next-Generation Sequencing (Ngs)
• Molecular Cloning
• Science
• Rnai
• Western Blotting
• Molecular Biology
• Northern Blotting
• Rna Isolation
• Rna Biology
• Pcr
• Dna
• Microscopy
• Biochemistry
• Microbiology
• Real-Time Polymerase Chain Reaction (Qpcr)
• Elisa
• Cell Biology