Kalpesh Gupta

WORK HISTORY

EDUCATION

ABOUT KALPESH GUPTA

Highly skilled and motivated Automation Enginner with 14+ years of industry experience in laboratory automation as applied to Molecular Biology, Cell Culture, Protein BiochemistryLeads company wide automation implementation to streamline process and increase efficiency Skills : Automation and Robotics: Developing and programing methods for different liquid handling platforms such as STAR, STARlet, Nimbus, ATOLL, Phynexus, TECAN.Protein chemistry: Optimization of methods for the purification of recombinant antibodies, glycoprotein, bispecific constructs, Fc fusion proteins, antigens and affinity-tagged (6x His-tag) proteins from E. coli and Mammalian cells using affinity (Protein A, Protein G and IMAC) and preparative SEC methods (AKTA Purifier), spectrophotometric quantitation of protein samples Protein Spectroscopic analysis, SDS-PAGE, IEF, Western Blotting, Size Exclusion Chromatography, high performance liquid chromatography (HPLC), Differential Scanning Calorimetery (DSC), ELISA, dissolution, Activity and Cell based assays, DLS, capillary electrophoresis.Molecular biology: standard DNA manipulation methods including conventional cloning (restriction enzyme digestion, agarose gel electrophoresis, gel purification and ligation of DNA fragments, bacterial transformation), ligation independent cloning (Novagen), construction of expression vectors, maxipreps, minipreps, colony PCR, PCR, DNA sequencing (Applied BioSystems 3100 sequencer) and quantitation (Nanodrop and Labchip DS).Bioinformatics : Systematic training in Computation Science and Bioinformatics. Proficient in using various Bioinformatics software and software development by using Python, Java, Perl, Google App Script.