Jifa Zhang
Associate Scientist @Sutro Biopharma, Inc.
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WORK HISTORY
Associate Scientist @Sutro Biopharma, Inc.
South San Francisco, CA, US
Development of chromatographic assays (reverse-phase HPLC, SEC, ProL, etc.) and LC-MS methods for the characterization of antibodies, cytokines, and antibody-drug conjugates-Establishment of LC-MS methods for the analysis of intact/reduced antibodies, antibody-drug conjugates, drug-antibody-ratio (DAR), and free thiols-Development of LC-MS methods for peptide mapping for single variant analysis;
EDUCATION
Peking Union Medical College
Master's degree, Medicinal Chemistry
Shandong University
Bachelor's degree, Pharmaceutical Sciences
Purdue University
Doctor's Degree, Analytical Chemistry
ABOUT JIFA ZHANG
A self-motivated, goal-oriented, experienced mass spectrometry specialist, analytical chemist, and medicinal scientist with extensive research experience in-developing chromatographic assays (reverse-phase HPLC, SEC, ProL, etc.) and LC-MS methods for the characterization of antibodies, cytokines, and antibody-drug conjugates-developing LC-MS methods for the analysis of intact/reduced antibodies, antibody-drug conjugates, drug-antibody-ratio (DAR), and free thiols-developing LC-MS methods for peptide mapping for single variant analysis-analysis of biomass degradation mixtures using LC-MS/MS-targeted protein degradation (proteolysis targeting chimera (PROTAC)), chemical proteomics, quantitative proteomics-fragmentation mechanisms of lignin model compounds upon CAD in the mass spectrometer-drug discovery, natural product modifications and synthesis. Skills and techniques: Mass spectrometry | HPLC-MS/MS | Intact antibody/antibody-drug conjugates analysis | free thiol quantification using LC-MS | Single variant analysis (SVA)| DAR analysis | Bioconjugation of antibodys and drugs | HPLC-HRMSn | fast pyrolysis coupled with HRMS | chemical proteomics | medicinal chemistry | natural product modifications | natural product synthesis Targeted protein degradation | Chemical probe design | protein extraction | click chemistry | affinity enrichment | protein digestion | post-translational modification analysis | mammalian and yeast cell culture | SILAC | TMT labeling | Proteomics database searching using Protein Matrix (Intact, reduced, ADC, Byos, Byosphere etc.) Sequest, MSFragger, Byonic, etc. Instruments: • Sciex ZenoTOF 7600 mass spectrometer • Agilent qTOF 6530 mass spectrometer • Thermo scientific Q Exactive Plus mass spectrometer • Thermo scientific linear quadrupole ion trap (LQIT) coupled with Orbitrap Elite (or XL) mass spectrometer (LQIT-Orbitrap mass spectrometer) • Thermo scientific linear quadrupole ion trap (LQIT) mass spectrometer • Thermo Dionex UltiMate UHPLC coupled to LQIT-Orbitrap Elite mass spectrometer • Agilent 1260 Infinity Quaternary HPLC coupled to LQIT-Orbitrap XL mass spectrometer • Thermo scientific Vanquish Duo UHPLC systems • Teledyne CombiFlash EZ PREP UV/ELSD HPLC Flash Chromatography • Agilent Technologies 1260 Infinity HPLC • BioMATE 3S UV-Visible spectrophotometer • Optima XPN-100 Ultracentrifuge
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