James Partridge

About James Partridge

Structural biology and biophysics.Select Publications-Dufu, K, Alt, C, Strutt, S, Partridge, J.R, Tang, T, Siu, V, Liao-Zou, H, Rademacher, P, Williams, A.T, Muller, C.R, Geng, X, Patel Pochron, M, Nguyen Dang, A, Cabrales, P, Li, Z, Oksenberg, D, Cathers, B.E.(2023). GBT021601 improves red blood cell health and the pathophysiology of sickle cell disease in a murine model. British Journal of Hematology. DOI: J.R, Choy, R.M, Silva-Garcia, A, Yu, C, Li, Z, Sham, H, and Metcalf, B.(2019). Structures of full-length plasma kallikrein bound to highly specific inhibitors describe a new mode of targeted inhibition. J. Struct. Biol. doi: https://doi.org/10.1016/j.jsb.20•••••01-Dementiev, A, Silva, A, Yee, C, Li, Z, Flavin, M.T, Sham, H, and Partridge, J.R.(2018). Structures of human plasma β-factor XIIa cocrystallized with potent inhibitors. Blood Advances. doi: https://doi.org/10.1182/bloodadvances.20••••••37-Li, Z, Partridge, J.R, Silva-Garcia, A, Rademacher, P, Betz, A, Xu, Q, Sham, H, Hu, Y, Shan, Y, Liu, B, Zhang, Y, Shi, H, Xu, Q, Ma, X, Zhang, L,(2017). Structure-Guided Design of Novel, Potent, and Selective Macrocyclic Plasma Kallikrein Inhibitors. ACS Med. Chem. Lett. 8, 185–190. doi:10.1021/acsmedchemlett.6b00384-Partridge, J.R.*, Lavery, L.A.*, Elnatan, D, Naber, N, Cooke, R, Agard, D.A,(2014). A novel N-terminal extension in mitochondrial TRAP1 serves as a thermal regulator of chaperone activity. eLife 2014;3:e03487.*equal contribution-Partridge, J.R.*, Lavery, L.A.*, Ramelot, T.A, Elnatan, D, Kennedy, M.A, Agard, D.A,(2014). Structural Asymmetry in the Closed State of Mitochondrial Hsp90 (TRAP1) Supports a Two-Step ATP Hydrolysis Mechanism. Molecular Cell. vol. 53 (2) pp. 330-343.*equal contributionSpecialties: Recombinant protein expression / purificationProtein engineeringCrystallization/structural analysis of heteromeric protein complexesCrystallography robotics: ArtRobbins Phoenix system Formulatrix Formulator, Rock ImagerCrystallographic data collectionProcessing and refinement of crystallographic data SAD/MAD/MR techniquesAUCITCCDFPLC/HPLCE. coli research tools/techniquesS. cerevisiae propagation/research techniquesFluorescence, Deconvolution, and Confocal microscopy.

Experience

1. Structural Biology

   Novartis

   Feb 2024 — Present · Emeryville, CA, US

Education

• University of California, Davis

  BS, Cell Biology

  1999 — 2004

• Massachusetts Institute of Technology

  Ph.D. degree, Biochemistry and Structural Biology

  2004 — 2010

Skills

• Recombinant Dna Technology
• Molecular Biology
• Itc
• Cell Culture
• X-Ray Crystallography
• Protein Engineering
• Chemistry
• Biophysics
• Cell Biology
• Fluorescence
• Structural Biology
• Enzyme Kinetics
• Biochemistry
• Protein Chemistry
• Analytical Ultracentrifugation
• Life Sciences
• Enzyme Assays
• Protein Characterization
• Protein Purification
• Purification
• Protein Expression