Ishita Choudhury

About Ishita Choudhury

Senior Research Associate with 4 plus years of experience in academia and industry in the skills listed below:• Construction, cloning and recombination of plasmids used in processes for building gene therapy molecules/vectors.• Conducting post-production assays like genetic structural assays, expression assays, spectrophotometric assays, aggregation assays, critical to the testing of our gene therapy adenovectors.• Responsible for the maintenance of cell lines whenever required by the department.• Next-generation sequence analysis of the adenoviral vector DNA.• Design and optimization of the new assays that are being introduced in the lab.• Handling operations in controlled GMP work environment and performing validation assays for Quality Control department.• Writing and editing detailed SOP’s and reports for GMP controlled experiments.•Designing experiments and performing validation assays on our gene therapy materials before release.• Extensive skills in different softwares like Gene construction kit and Geneious.Along with these skills I have previous experience in:•DNA and RNA isolation from murine myocardium samples. •Experience in different staining techniques like H&E staining and Mason\'s trichrome staining on tissue sections.•Performed qRTPCR, FACS staining, and western blotting from the cells harvested from mouse lung tissue.•Maintained the lung cancer cell line LLC according to the ATCC standards.•Isolated RNA from the murine cells and conducted agarose gel electrophoresis, gene expression studies, coculture studies, invasion and migration assays and FACS staining.

Experience

1. Associate Scientist

   BioNTech SE

   Oct 2023 — Present · Gaithersburg, MD, US

Education

• University of Allahabad

  Bachelor of Applied Science (B.A.Sc.), Applied Science

  2009 — 2012

• Vellore Institute of Technology

  Master's degree, Biotechnology

  2012 — 2014

• University of Bridgeport

  Master’s Degree, Biomedical/Medical Engineering

  2016 — 2018

Skills

• Cell Signaling
• Cell Culture
• Bioinformatics
• Nanoparticles
• Facs
• Cancer Biology
• Xml
• Cell Based Assays
• Molecular & Cellular Biology
• Statistics
• Stem Cell Research
• Sds Page
• Elisa
• Biochemistry
• Sds-Page
• Immunoassays
• Biotechnology
• Protein Purification
• Stem Cells
• Uv-Vis
• Immunology
• Tissue Engineering
• Flow Cytometry
• Qrt-Pcr
• Data Analysis
• Polymerase Chain Reaction (Pcr)
• Research
• Lifesciences
• Microsoft Office
• Western Blotting
• Matlab
• Tumor Immunology
• Gel Electrophoresis
• Biology
• Project Management
• Powerpoint
• Molecular Biology
• Cancer Research
• Uv/Vis Spectroscopy
• Comsol