Ishankumar Soni
Protein Biochemistry | Molecular Biology | Structural Biology | Antibody/ Antigen Engineering and Production | Therapeutic Antibody and Protein Vaccine Development
- Role
- Scientist Iii, Downstream Purification Development at Thermo Fisher Scientific
- Location
- Tewksbury, MA, US
- LinkedIn followers
- 500 followers
About Ishankumar Soni
I am a Ph.D. scientist with 10+ years of experience (3+ years of industrial experience) in protein science - protein biochemistry, molecular and structural biology.• My multidisciplinary skillset include- Protein Expression (bacterial expression system: E. coli, mammalian expression systems: CHO and HEK, and stable cell line generation via lentiviral particles)- Mammalian Cell Culture (Suspension and Adherent Cell Lines)- Protein Purification using FPLC: protein A affinity chromatography, immobilized metal-ion affinity chromatography (IMAC), strep-tag affinity chromatography, size exclusion chromatography (SEC), ion exchange (AEX and CEX) chromatography, and using AKTA Pure - TFF, SEC-MALS, Octet BLI, ELISA, FACS, SDS-PAGE, Western Blots, luciferase assays, and virus neutralization assays - HPLC (analytical-SEC and HIC)- Protein Engineering: PCR, Site-Directed Mutagenesis, Gibson Assembly, Primer Designing, Maxi Prep, Mini Prep, and Agarose Gel Electrophoresis - Enzyme Kinetics (Spectramax Microplate Reader)- HDX-MS (Waters Synapt G2Si Q-TOF, nanoACQUITY UPLC, PLGS, and DynamX)- Fluorescence Polarization/ Anisotropy (FITC Labeling)- DSC and DSF
Experience
Scientist Iii, Downstream Purification Development
Mar 2026 — Present · Bedford, MA, US
Education
Kutztown University of Pennsylvania
Bachelor of Science (B.S.), Chemistry - Analytical Chemistry Concentration
2012 — 2014
University of Massachusetts Amherst
Doctor of Philosophy (Ph.D.), Chemistry - Biochemistry Concentration
2015
Lehigh Carbon Community College
Associate of Science (A.S.), Natural Sciences
2010 — 2012
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