Igor Dangelo

About Igor Dangelo

Scientist experienced in rational protein and antibody engineering, molecular modeling, therapeutic modalities, functional assays and preclinical formulation. • Expert in engineering antibody-antigen interactions and improving stability using in silico predictive mutagenesis, in vitro maturation or a combination of the two.• Developed several bi- and multi-specific protein and antibody scaffolds targeting different applications (i.e. autoimmunity, inflammation, cancer)• Expert in designing new therapeutic modalities to optimize binding, potency and modulation of the immune system effector function.• Routinely engaged in analyzing data from biochemical and biophysical characterization.• Experienced in cloning, mutagenesis, expression in bacterial and mammalian cell lines and purification.• Experienced in deep sequencing to improve library screening and facilitate structure-based engineering.• Familiar in designing pre-formulation assays to enable downstream PK/PD, toxicity and GMP/GLP activities.• Familiar with the identification and development of in vitro, ex vivo and in vivo models for characterizing mechanism of action and therapeutic window. • Familiar with modeling software (Schroedinger, Rosetta, MOE, etc) and statistical analysis.• Experienced in protein and antibody construct optimization, crystallization and crystal structure determination and analysis.• Experienced team leader, planned, enabled and successfully completed several projects at Amgen and Zymeworks (also in collaboration with Merck, Eli-Lilly and Celgene). • Multi-year experience managing resources and cross-functional collaborations with CROs and business partners. • Experienced in critically assessing projects, developing efficient workflows, formulating new ideas and providing scientific direction to increase success. • Authored several peer-reviewed publications and contributed to invention disclosures for patent applications.

Experience

1. Antibody Engineering

   Lawrence Livermore National Laboratory

   Sep 2024 — Present · Thousand Oaks, CA, US

Education

• Università di Pavia

  BSc/MSc, Biological Sciences

  1995 — 2000

• EMBL-Heidelberg

  PhD, Molecular and Structural Biology

  2000 — 2004

• Heidelberg University

  PhD, Biochemistry

  2000 — 2004

Skills

• Genetics
• Proteomics
• Cell Based Assays
• Pk/Pd
• Elisa
• Antibodies
• Characterization
• Crystallography
• Structural Biology
• Immunohistochemistry
• In Vivo
• Flow Cytometry
• Molecular Cloning
• Mass Spectrometry
• Biochemistry
• Cell Biology
• Drug Discovery
• Molecular Dynamics
• Confocal Microscopy
• Spr
• Cell Culture
• Bioinformatics
• X-Ray Crystallography
• Laboratory
• Western Blotting
• Molecular Biology
• Hybridoma
• Computational Biology
• Purification
• Protein Expression
• Hplc
• Protein Chemistry
• Lc-Ms
• Genomics
• Life Sciences
• Protein Engineering
• Antibody Engineering
• Biotechnology
• Protein Purification
• Molecular Modeling