Hasitha Premathilake
Automated High Throughput Cell Culture | hiPSC culture | 3D Cell Culture | Organoid Development | NGS Bioinformatics | Metagenomics | Stem-Cell Dynamics | Molecular Cell Biology
- Role
- Biological Scientist - Automated Cell Culture Expert Group (Card) at National Institute On Aging Nia
- Location
- Frederick, MD, US
- LinkedIn followers
- 500 followers
About Hasitha Premathilake
An accomplished scientist with 6+ years’ experience in utilizing molecular biology and…
Experience
Biological Scientist - Automated Cell Culture Expert Group (Card)
National Institute On Aging Nia
Oct 2024 — Present · Bethesda, MD, US
Bring greater optimization, reliability, and reproducibility to the culture, maintenance, and differentiation of hiPSCs using a high-throughput automated cell culture system based on Hamilton Microlab VANTAGE automated multimodal reagent dispenser platform, thus producing efficient and reproducible workflows.Simultaneous cultivation of multiple cell lines that can be differentiated into different cell types (neurons, microglia and astrocytes) for downstream assays, through automated hiPSC introduction, single-cell dissociation, transfection, and long term differentiation into neurons, microglia and astrocytes (roughly 28 days).Serving as the back born of the iPSC Neurodegenerative Disease Initiative (iNDI) at the Center for Alzheimer\'s and Related Dementia (NIA/NIH), responsible for the high-throughput automated expansion and differentiation of a multitude of iNDI cell lines (containing ADRD related mutations) for downstream high-throughput genomic, transcriptomic, and proteomic analysis.Championing the implementation of laboratory best practices for reduced contamination and operational cost in a fully automated state-of-the-art laboratory environment.
Education
University of Colombo
Bachelor's degree, Bioinformatics
2007 — 2011
Oklahoma State University
Doctor of Philosophy - PhD, Animal Sciences
2017 — 2021
Nalanda College, Colombo.
Biological Science
1997 — 2005
Cardiff Metropolitan University
Master of Business Administration (M.B.A.), General MBA
2013 — 2014
University of South Dakota
Doctor of Philosophy - PhD, Bioinformatics (reading)
2016 — 2017
SLIIT
Bsc. Special Honours, Information Technology
2006 — 2010
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