Hasitha Premathilake

Automated High Throughput Cell Culture | hiPSC culture | 3D Cell Culture | Organoid Development | NGS Bioinformatics | Metagenomics | Stem-Cell Dynamics | Molecular Cell Biology

Role
Biological Scientist - Automated Cell Culture Expert Group (Card) at National Institute On Aging Nia
Location
Frederick, MD, US
LinkedIn followers
500 followers

About Hasitha Premathilake

An accomplished scientist with 6+ years’ experience in utilizing molecular biology and…

Experience

  1. Biological Scientist - Automated Cell Culture Expert Group (Card)

    National Institute On Aging Nia

    Oct 2024 — Present · Bethesda, MD, US

    Bring greater optimization, reliability, and reproducibility to the culture, maintenance, and differentiation of hiPSCs using a high-throughput automated cell culture system based on Hamilton Microlab VANTAGE automated multimodal reagent dispenser platform, thus producing efficient and reproducible workflows.Simultaneous cultivation of multiple cell lines that can be differentiated into different cell types (neurons, microglia and astrocytes) for downstream assays, through automated hiPSC introduction, single-cell dissociation, transfection, and long term differentiation into neurons, microglia and astrocytes (roughly 28 days).Serving as the back born of the iPSC Neurodegenerative Disease Initiative (iNDI) at the Center for Alzheimer\'s and Related Dementia (NIA/NIH), responsible for the high-throughput automated expansion and differentiation of a multitude of iNDI cell lines (containing ADRD related mutations) for downstream high-throughput genomic, transcriptomic, and proteomic analysis.Championing the implementation of laboratory best practices for reduced contamination and operational cost in a fully automated state-of-the-art laboratory environment.

Education

  • University of Colombo

    Bachelor's degree, Bioinformatics

    2007 — 2011

  • Oklahoma State University

    Doctor of Philosophy - PhD, Animal Sciences

    2017 — 2021

  • Nalanda College, Colombo.

    Biological Science

    1997 — 2005

  • Cardiff Metropolitan University

    Master of Business Administration (M.B.A.), General MBA

    2013 — 2014

  • University of South Dakota

    Doctor of Philosophy - PhD, Bioinformatics (reading)

    2016 — 2017

  • SLIIT

    Bsc. Special Honours, Information Technology

    2006 — 2010

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Hasitha Premathilake — Biological Scientist - Automated Cell Culture Expert Group (Card) at National Institute On Aging Nia in Frederick, MD, US | Unifers