Harold Tapamo
Scientist/Engineer
- Role
- Car-t Cell Scientist at Eurofins
- Location
- Philadelphia, PA, US
- LinkedIn followers
- 500 followers
About Harold Tapamo
I have dedicated my career to medicine, research, and community service. CAR-T…
Experience
Car-t Cell Scientist
Oct 2022 — Present · Ambler, PA, US
Automation of the process for ordering new material and requesting material in inventory. Reduces the time to formulate newmaterial by 30% and reduced the time to retrieve stored material by 50%.• Formulation of apheresis material following the established protocol for over 50 donors, creating 150+ high density bags.• Coordinates with groups on maintaining and distributing 25+ different patients’ apheresis material in liquid nitrogen.• Provides apheresis support for CARVYKTI. enabling studies with shared/repeat APHs, managing inventory for future studies & long-term stability.• Contributing technical and clinical expertise through a technical understanding of immunology and industry sources• Seeding, Expansion, Transduction, and Harvest of healthy donor (human) cell cultures supporting CARVYKTI and new drugtargets.• Maintaining cell line for studies that range from 11 days to 3 months, proficient in aseptic technique• Develop cell lines for cGMP biologic therapeutics for late stage clinical projects• Assists in enrichment, activation, transduction, and harvest of T-Cells using Miltenyi Biotec Prodigy/GREX100(CS) for five ofour process teams.• Ordering healthy donor material including Leukopaks from AllCells, Hemacare, and Charles River Laboratories. Purchase Ordergeneration and execution, cost management, and delivery management.• Manages liquid nitrogen inventory of >180 healthy donor apheresis material consisting of >220 bags for department use.• Cell viability assays using Nucleocount NC-200 and XM-30 along with Complete Blood Count using DxH 500• Cell composition (8 C & 3C) and memory phenotype flow cytometry performed on MACsQuant and Accelix
Education
University of Miami
Bachelor of Science - BS, Bioengineering and Biomedical Engineering
2014 — 2018
University of Miami Miller School of Medicine
Medicine
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