Gayathri Ganesh

WORK HISTORY

EDUCATION

ABOUT GAYATHRI GANESH

I am an immunology and stem cell scientist. I have two masters degrees - MMSc in immunology from Harvard University and MS in physiology from State University of New York. I provide scientific, technical support and project management services to pharmaceutical clients. Feel free to connect via email g••••••••@live.com. Background:Worked in microbiology, immunology - autoimmunity, wound healing, stem cell and neuroscience labs across the world, in the USA, India and Middle East. My research, project management and collaborations resulted in 2+ publications in high impact journals and 7+ conference presentations and awards. 6+ years laboratory experience with expertise in range of technical and analytical skills:Cell Culture:• Cell Culture – Bacterial, iPSC, neutrophils, macrophages• Cell differentiation – Neural progenitor, Neurons• Mouse Primary cell isolation – bone marrow, blood, immune cellsCell based and Functional assays:• Cell Counting & Viability – MTS, Fluorescence assay• Cell culture QC – Mycoplasma test• Cell proliferation – EdU, Ki67• Handling human plasma samples• Magnetic sorting• Extracellular Vesicles/Exosome isolation • Ultracentrifugation• Nanotracking analysis • Immunofluorescence/Immunocytochemistry• Live cell imaging• Flow cytometry• Spectral cytometryHistology:• Skin tissue sectioning• H&E stainingMolecular Biology:• RNA isolation • cDNA isolation• Primer Design • RNA quality – Qubit and Bioanalyzer• Protein extraction and quantification• qPCR, RT-qPCR – 96, 384 well• ELISA• SDS-PAGE• Western Blot• RNA Sequencing Analysis Software:• MS Office – Word, Excel, PowerPoint, Outlook, Teams• Project management - Asana• ELN - Lab archives, OneNote• R Studio• Graph Pad Prism• Flowjo• Spectral flo• Molecular Devices Pico Express• ImageJ• Image Lab• Primer Blast/Primer3/Primer Quest• CFX Maestro