Lawrence William

About Lawrence William

I love biology and chemistry because it give us an insight into how life works.• Process Development Associate Scientist focused on designing, developing and qualifying analytical and CHO host cell protein impurity methods for drug discovery under Internal company and ICH compliance.• Developed CHO host cell protein methods for supporting and advancing late phase drug discovery and biosimilars. • Proven success in pre-clinical assay development with excellent working knowledge of analytical chemistry, ECL and ELISA immunoassay formats.• Wrote for peer review technical documents which include protocols, SOP’s, validation plans, study forms, data reports, and contributed to IND and BLA filing. • Expertise includes protein purification, HPLC analytical methods, critical reagents, ELISA, Immunoassay development, and technical documents.

Experience

1. Scientist Potency and Functional Characterization

   百时美施贵宝

   Jan 2021 — Present · Seattle, WA, US

   Bristol Meyers Squibb, Seattle, WA 2021 - Present Scientist | Product Potency & FunctionAnalytical Development lead responsible for development and qualification of healthy donor vector potency bioassay method for vector characterization, and vector potency by reporter cells for lot release. Developed Allogeneic CRSPR CAS9 CD19 transgene expression assay. Assisted with CAS9 and CAS12a cell line engineering and clone selection. Assisted in developing liposome gRNA transfection and transduction procedures. Write qualification plan and method development reports. • CD4+, CD8+ donor T cells, Jurkat nur77 and NFAT reporter cells transduction • Activate effector cells with antigen specific target cells and anti-idiotype antibody.• Cytokine secretion quantitation by AlphaLISA immunoassay• Luciferase reporter cell vector potency• Completed GPCRD program and assisted with Breyanzi and Abecma potency assays.• Allogeneic CAS9 CD19 transgene expression assay• Transferred and trained QC on CRISPR CAS9 and CAS12a transgene assay• CD4+, CD8+ donor T cells, Jurkat nur77 and NFAT reporter cells transduction • Activate effector cells with antigen specific target cells and anti-idiotype antibody.• Cytokine secretion quantitation by AlphaLISA immunoassay• Luciferase reporter cell vector potency• Completed GPCRD program and assisted with Breyanzi and Abecma potency assays

Education

• Northern Michigan University, Marquette, MI

  B.S., Bachelor of Science, Biology, Group Science

• Northern Michigan University

  B.S., Bachelor of Science, Biology, Group Science

Skills

• Immunoassays
• Proteomics
• Elisa
• Process Qualification
• Affinity Chromatography
• Sec
• Jmp
• Affinity Purification
• Chromatography
• Protein Conjugation
• Drug Discovery
• Hplc-Ms
• Cell Culture
• Gel Electrophoresis
• Drug Development
• Western Blotting
• Biopharmaceuticals
• Sop
• Ion Exchange
• Purification
• Hplc
• Protein Chemistry
• Life Sciences
• Hcp
• Biotechnology
• Protein Purification
• Monoclonal Antibodies
• Assay Development