Piyush Sethia
Group Leader - Molecular Biology @PI Industries Ltd
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WORK HISTORY
Group Leader - Molecular Biology @PI Industries Ltd
Udaipur, IN
Building capability to perform cutting-edge molecular biology work for enzyme engineering towards achieving larger business goals of the organization in biotech, agritech, small-molecules space• Designing strategies and working promptly in collaboration with various cross-functional teams in a matrix organizational structure towards generating robust microbial industrial platform strains
EDUCATION
Indian Institute of Technology, Bombay
Doctor of Philosophy (Ph.D.), Oxidative stress; Promoter studies; Protein expression
SIES College, Sion (University of Mumbai)
Master of Science (M.Sc.), Microbiology
Swami Vivekananda College, Chembur (University of Mumbai)
Bachelor of Science (B.Sc.), Microbiology
SKILLS
ABOUT PIYUSH SETHIA
Technical Expertise:• Analysis and targeted modifications of cellular metabolic network in the genomes of P. pastoris, E. coli, B. subtilis, Ralstonia eutropha, Cyanobacterium sp, Pseudomonas sp. and Clotridium sp.• Molecular Biology - CRISPR, Gibson and Infusion assembly, RNA seq, RT-PCR, promoter studies, creating SDM’s, reporter fusions, PTM’s, plasmid addiction, auxotrophy based selection strategies• Microbiology, Biochemistry, Bioprocess Technology and Bioinformatics - Microbial growth monitoring and Scale-up, DSP, Enzyme assay, Native/SDS-PAGE, EMSA, Western Blotting, Zymogram analysis• High Cell Density Fermentation - Operation of a lab scale bioreactor in batch and fed-batch modes• Chromatographic techniques including immobilized metal ion affinity chromatography (IMAC), Heparin Sepharose chromatography and Ion Exchange chromatography• Well verse with literature analysis, documentation, industrial safety guidelines, team management
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