Ashish

About Ashish

As a Research Scientist B at the ICMR DHR – Multidisciplinary Research Unit, Department of Anatomy, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India, I have gained valuable experience and expertise in the field of Molecular Biology and Medical Genetics. With a strong dedication to my work and a diligent approach, I have been an integral part of the Covid-19 Diagnosis and research team, making significant contributions to the field.One of my notable achievements includes receiving BSL2 training for COVID-19 testing, enabling me to actively contribute to the testing and diagnosis of the disease. Over the course of two years, I have been extensively involved in performing Whole-genome sequencing using nanopore technology such as MinION/GridION Oxford Nanopore and Illumina MiSeq. Additionally, I have been responsible for conducting RT-PCRs and viral RNA extraction. Through these experiences, I have gained a comprehensive understanding of the various laboratory instruments involved in these processes, as well as troubleshooting techniques.My research work has been primarily focused on the genetics basis of infertility in females due to endometriosis. This clinically driven investigation involved laparoscopic examinations and questionnaire-based surveys to record patients\' lifestyle, habits, and familial history. I have conducted molecular genetics investigations to study different genes associated with endometriosis in both familial and sporadic cases. By understanding the genetic and epigenetic pathways involved in the progression of endometriosis, I have successfully validated different biomarkers for the diagnosis of the condition. Notably, I have explored the transition from hormonal to endometrial cyst form to effectively examine, classify, and treat patients, ultimately aiming to prevent the disease from becoming more severe. Throughout these studies, I have utilized various molecular biology techniques such as rt-PCR, Exome sequencing, and Sanger sequencing. Fluorogenic probes specific to target genes have been employed to detect particular genes involved in the disease, and epigenetic studies have involved calculating copy numbers and analyzing relative gene expression levels. Additionally, I have utilized RNA seq GEO dataset analysis to predict potential lncRNAs as biomarkers for endometriosis.

Experience

1. Research Fellow (Phd) in Medicine

   Banaras Hindu University

   Sep 2017 — Present · Varanasi, IN

   Implication of preclinical biomarkers in identification of mild cognitive impairment (MCI) and Alzheimer’s disease: Promises and future challenges.Mild cognitive impairment (MCI) has attracted central attraction by various researchers to diagnose prodromal stage of Alzheimer’s disease (AD) by using different biological and imaging biomarkers. In evaluating disease changes, it is critical to have measurements that are sensitive, specific, and reliable. The purpose of this review is to bring a comprehensive overview about the recent studies done in development of biomarker for clinical diagnosis of MCI and pre-AD using different technological interventions. The current state of knowledge highlights the implication of certain proteins, transporter, enzymes and metabolic pathway related factors, radiotracer in identification of disease progression. However, all these studies done on biomarker development lack sensitivity and specificity to be classified as clinical marker for MCI and AD. The revised criteria of NIA-Alzheimer’s association in diagnosis of MCI, pre-clinical AD and AD encompass use of biomarker which can detect pathological process and subsequent neurodegeneration. The present article highlights the significant development in field of plasma, cerebrospinal fluid, serum and neuro imaging biomarkers which have shown positive result in various studies and the future challenges in application of these biomarkers at clinical setting in early intervention of disease.

Education

• Banaras Hindu University

  Doctor of Philosophy - PhD, Medicine

  2017

• Kanpur University, India

  Bachelor's degree, Biotechnology

  2011 — 2015

• Indian Institute of Technology, Guwahati

  Data Science , Credit-Linked Program in Data Science