Denis Arutyunov

WORK HISTORY

EDUCATION

SKILLS

ABOUT DENIS ARUTYUNOV

& hl=enBiochemist (enzymologist and protein scientist), cell and molecular biologist.Discovered new DNA binding and pathogen capturing proteins.Taught undergraduate courses, trained and supervised junior colleagues. Led collaborative projects, co-authored US patents.Skilled in- Genetic manipulations: construct design, molecular cloning, recombinant gene expression, qPCR, mutagenesis, animal cell transfection, bacterial strain engineering- Protein research: stability and protein-ligand interaction analysis (ELISA, PAGE, WB, IP, AUC, DSC, SPR, MS, CD, UV-Vis-fluorescence spectroscopy), crystallization and high throughput robotic screens, enzyme kinetic assays, chemical synthesis and modification procedures, production and characterization of antibodies- Purification methods: lysis, extraction, IMAC, SEC, IEX, HIC, AKTA FPLC / Unicorn, HPLC, TFF, dialysis / diafiltration, lyophilization- Cell culturing: bacterial, yeast, insect and human cells. Anaerobic and fermentation / bioreactor procedures- Viral systems: bacteriophages, baculoviral and lentiviral expression systems- Cell-based assays: proliferation and toxicity, FACS, electrochemical diffusion chamber (Ussing) and fluorometric perfusion techniques- Microscopy: electron (including immunogold), fluorescence (wide-field and confocal) including live cell imaging procedures- Animal work: rabbit immunization, express blood analysis, metabolic cages, handling and perfusion of mice, tissue dissecting, primary cell cultures- Handling of biohazard materials including clinical specimens and biosafety level 2 pathogens- Handling of radioactive isotopes including gamma emitters, e.g, radioiodine- Handling of highly toxic chemicals, e.g, bromine and cyanides- Bioinformatics: biomedical databases, alignment and topology prediction engines, statistical software (JMP, Prism), main R packages and Python libraries.