David Mikles

About David Mikles

Molecular Biologist with 5 years of experience in manufacturing IVD products in a biotech start-up and scientific leadership roles for Assay Development in a fast-paced client-driven manufacturing organization, and 4 years postdoctoral experience. Highly motivated and versatile as demonstrated by achieving milestones across 10 viral vector projects covering AAV, lentivirus, and baculovirus-Completed Milestones within set time-frames and budget-Experienced with AAV, lentivirus, and baculovirus for GMP production-Supervised 4-20 colleagues daily activities over the years-Drove the development of 5 individuals and promotion of one of my mentees-Developed multiple calendars to plan and track daily activities for multiple team members Increasing morale by reducing unplanned events and optimizing workload for reduced timelines-Developed single cell printing for HEK293 and SF9 cell lines-Reduced development timelines for baculovirus by 1 month-Developed high throughput screening for starting material testing using automated Liquid handlers-Improved cell maintenance through reducing costs by 20% and reducing workload by 30%.Technical skills:Cell Based-Upstream: transformation, transfection, harvest, Ambr bioreactor, hyper stack scaling-Assays: rcAAV, infectious assays, cell Line adaptation, single cell cloning and residual infectious assays.DNA Quantification and Design-qPCR, ddPCR, Residual DNA assays and molecular cloning.Protein Characterization and Activity-Downstream: Affinity, ÄKTA, TFF-Assays: fluorescence, dose-dependent Inhibition, UV/Visible spectroscopy, AUC, SDS-PAGE, ELISA, western blot, circular dichroism, mass spectrometry, multi-angle static and dynamic light scattering.Software-PowerPoint, Prism, Visio, Smartsheet, SharePoint, JMP, Word and Outlook.Soft skills: problem solving, analytical thinking, self-motivation, perseverance, creative thinking, excellent oral & written communication, data interpretation, project & time management, cross-functional collaboration, stress management, flexibility.

Experience

1. Post Market Surveillance Specialist Iii

   Thermo Fisher Scientific

   Aug 2024 — Present · Los Angeles, CA, US

Education

• University of Miami Miller School of Medicine

  Doctor of Philosophy (PhD), Philosophy

  2010 — 2014

• University of Arizona

  Bachelor of Science, Molecular and Cellular Biology

  2007 — 2009

Skills

• Molecular Biology
• Polymerase Chain Reaction (Pcr)
• Programming
• Microsoft Office
• Research
• Pcr
• Data Analysis
• Cell Culture
• Higher Education
• Powerpoint
• Microsoft Excel
• Statistics
• Microsoft Word
• Matlab
• Public Speaking
• Teaching