Dan Yu

About Dan Yu

10-year research experience as scientist in Molecular Biology and Biochemistry in top university and research center. Deep knowledge and extensive hand-on experience in relevant biochemical topics in synthetic biology, metabolic engineering, enzymology, protein engineering and drug discovery.>Designed, conducted, and analyzed research experiments in molecular cloning, gene editing (micro RNA interference, CRISPR/Cas9) and metabolic pathway regulation of plants and microorganisms for valuable chemicals. Experienced in protein purification, in vitro enzymology assays, protein crystallization, protein engineering, protein-ligand interaction for drug discovery.>Productive researcher with over authored and co-authored publications.Experimental Skills:>Molecular and Cell biology: genomic DNA isolation and & estimation, RNA isolation & estimation, PCR, library preparation, plasmid construction, vector optimization, Gateway reaction for multiple gene expression, artificial microRNA interference for gene knockdown, CRISPR/Cas9 for gene knockout.Transformation, cultivation and feeding of E. coli, yeast and mammaline cell, Agrobacterium-mediated and particle bombardment transformation of plants>Biochemistry: protein expression (E. coli, yeast, mammalian cells), protein purification (Ni-AC, ion exchange, SEC), protein engineering (fusion protein, truncations, mutagenesis), in vitro enzyme kinetic assay and substrate specificity test, Isothermal Titration Calorimetry (ITC) for inhibitor-protein,DNA-protein,protein-protein binding affinity, EMSA, spectrohpotometry,protein crystallization and structure determination by X-ray crystallography>Microscopy: fluorescence microscope for protein subcellular localization characterization>Analytical Chemistry: TLC, HPLC, GC-MS, nano-EIS-MS/MS et al, for separation, analysis and quantification of small molecules.Computer Skills:Data anlysis by progrmming (Pymol, R) and statistics software (GraphPad Prism, SPASS ), Geneious, WMD3 amiRNA Designer, ImageJ, ChemicDraw, Adobe Illustrator and Photoshop,Microsoft Ofiice.Communication Skills:Good communicator in English and Mandarin Chinese, for research and daily communication.

Experience

1. Global Marketing Director

   Hzymes Biotech

   Aug 2025 — Present · Shanghai, CN

Education

• Northwest A&F University

  Bachelor’s Degree, Bioengineering

  2006 — 2010

• The University of Göttingen

  Doctor of Philosophy (Ph.D.), Biochemistry and Molecular Biology

  2013 — 2017

• Northwest A&F University

  Master’s Degree, Biochemistry

  2010 — 2013