Chidambaram Ramanathan

Scientist @Cirquest Labs Mlm Medical Labs

Memphis, TN, US
MOBILE NUMBERS
+91 *********19

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WORK HISTORY

Oct 2017 — Present

Scientist @Cirquest Labs Mlm Medical Labs

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Memphis, TN, US

EDUCATION

1997 — 2003

Madurai Kamaraj University

Doctor of Philosophy (PhD)

SKILLS

ImmunohistochemistryQpcrMicroscopyProtein ExpressionLife SciencesMolecular GeneticsData AnalysisAnimal ModelsScienceBiochemistryPcrRecombinant Dna Techniques Including Topo and Recombination-Based Gateway Clone• Lentiviral Vector Construction and Viral Particle PreparationWestern Blotting• Expertise in Genomic and Chemical Library High-Throughput Screening• Expertise in Cell Culture and Cell Biological ExperimentsResearchPolymerase Chain Reaction (Pcr)Real-Time Polymerase Chain Reaction (Qpcr)Molecular BiologyBiotechnology• Expertise in Bioluminescence Technology in Low and Throughput-Based AssaysBiologyGenetics•Avv & Lentiviral Injection in Specific Brain Regions Using Stereostaxic MethodCell Biology• Expertise in Rnai Methods (Sirnas & Shrnas) and Crispr/Cas9 Ko in Cell LinesCell CultureCell• Mouse Tail Vein Injection of Viral ParticlesMammalian Cell Culture

ABOUT CHIDAMBARAM RAMANATHAN

Innovative scientist with 15+ years of experience in Biological Sciences, Molecular Biology, Cellular & Mice Genetics, Neuroscience, and cell based assay development. I am passionate about scientific discovery and developing new and improved methods for understanding biological systems. In my current position, I developed lenti-and adenoviral vector delivery systems to engineer multiple human and mammalian cell lines as circadian clock reporters model systems. The resulting stable cell lines allow for real-time in vitro monitoring of circadian dynamics in high-throughput format. This data was instrumental to award $5 million grant for five years.My expertise includes cell culture systems and animal models to delineate molecular mechanisms. I have extensive experience with neurobiology, molecular biology and biochemistry methods such as molecular cloning, recombinant protein purification, qRT- PCR, ELISA development, flow cytometry, FACS, recombinant DNA techniques, high-throughput screening using genetic and chemical libraries, developing bioluminescence reporter cell lines, designing and employing a library of shRNA and siRNA, designing and employing CRISPR/Cas9 tool to generate knockout cell lines, cell based assays, gene therapy, and generation of mammalian primary cell lines (MEFs and tail fibroblasts). With proven skills in neurobiology, molecular biology, assay development and project management, my goal is to become part of a dynamic research group that focuses on scientific discovery and development of innovative products.Feel free to contact me. C••••••••@gmail.com

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Chidambaram Ramanathan — Scientist at Cirquest Labs Mlm Medical Labs in Memphis, TN, US | Unifers