Ling Fu
Principal Scientist, In Vivo Pharmacology/ Translational medicine/ IND submission
- Role
- Principal Scientist at Bolt Biotherapeutics, Inc
- Location
- San Francisco, CA, US
- LinkedIn followers
- 500 followers
About Ling Fu
Research Scientist with a Ph.D. in Immunology and 10 years post-graduated experience in industry and academia[PROFESSIONAL SKILLS](A) CELL BIOLOGY:a. Mammalian cell lines and primary cell cultureb. Isolation and purification of primary cells from mouse and human PBMC by MACS and sorterIMMUNOLOGICAL TECHNIQUESa. Immunofluorescence staining (surface and intracellular antigen staining), cell cycle stainingb. Histology: processing tissues, H&E & immunohistochemistry (IHC)c. Multiple parameter flow cytometry (BD certificate, FACSCalibur, LSRII, Fortessa) and cell sorter (Aria II operator certificate) d. ELISA, MSD, Luminex e. Cellular immune function assays, including MLR/proliferation/cytotoxicity/apoptosis assays.(B) ANIMAL STUDIES: a. Rodent survival surgery: bladder wall injection, transurethral catheterization, intra-tracheal injection b. In vivo disease models:(1) asthma animal model,(2) Schistosomiasis mouse model by bladder wall injection,(3) uropathogenic bacterial infection and (4) orthotopic bladder tumor mouse model by transurethral catheterization or bladder wall injection (5) syngeneic tumor model by subcutaneous inoculation.c. Mouse in vivo imaging:(1) bioluminescence imaging (IVIS 200),(2) ultrasonography d. Mouse handling skills and dissection, i.p. and s.c. injection(C) MOLECULAR BIOLOGY: PCR, q-PCR, gene cloning and, adenoviral vector construction, production and infection for gene therapy(D) COMPUTER SKILLS: BD FACSDiva, FlowJo, Microsoft Office (Word, Excel, PowerPoint and Outlook), GraphPad Prism, and Image J
Experience
Principal Scientist
Feb 2025 — Present · Redwood City, CA, US
Education
National Taiwan University
Master's degree, Immunology
National Taiwan University
Bachelor's degree, Agricultural Chemistry
National Taiwan University
PhD, Immunology
Skills
- Cell Biology
- Cell Culture
- Immunofluorescence
- Animal Models
- Western Blotting
- Immunohistochemistry
- Cellular Assays
- Laboratory Skills
- Laboratory
- Immunoassays
- Elisa
- Life Sciences
- Flow Cytometry
- In Vivo
- Molecular Biology
- In Vitro
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