Venkatesh Vasu Chari

About Venkatesh Vasu Chari

Dedicated and accomplished Research Scientist with more than 8 years of professional experience, seeking a dynamic and intellectually challenging research position, with an aim of applying my knowledge and skills towards targeted research to gain novel insights and to lead to the development of new therapies and/or biomarkers.Expertise: Molecular cloning, mammalian tissue culture techniques, DNA/RNA extraction, PCR, RNAi (siRNA/shRNA), Capillary Electrophoresis (CE), Sequence analysis. Worked extensively on Protein extractions, Protein estimations, SDS-PAGE, Western blot, ELISA, Protein-Protein interaction assays: Yeast/mammalian 2-hybrid systems, Immunoprecipitation (IP) and Co-IP. Protein-DNA interaction - ChIP and ChIP-Seq.Proficient with the use of fluorescent microscopy, Flow cytometry, cell proliferation assays, Apoptosis assays. Extensively experienced in small animal (mice) handling - Breeding, colony maintenance, intra-venous (IV), intra-peritonial (IP) and tail-vein injections, ear-tagging, tail snipping, genotyping, tumor cell inoculation, tumor measurement and tumor excision and analysis.Computer Tools: GraphPad Prism, FlowJo, SeqScanner, SeqScape, JMP basics, MS Word, Excel, Powerpoint. Windows/Mac proficient.

Experience

1. Market Development Manager

   Eurofins DiscoverX

   Jan 2023 — Present · Fremont, CA, US

Education

• The University of Toledo

  Doctor of Philosophy (Ph.D.), Oncology and Cancer Biology

  2008 — 2013

• University of Mumbai

  Bachelor of Science (B.Sc.), Biotechnology

  2003 — 2006

• University of Mumbai

  Master of Science (M.Sc.), Biotechnology

  2006 — 2008

Skills

• Protein Assays
• Agarose Gel Electrophoresis
• Drug Discovery
• Pbmc Isolation
• Elisa
• Chromatin Immunoprecipitation
• Cell Viability Assays
• Reverse Transcription
• Immunostaining
• Fluorescence Microscopy
• Apoptosis
• Flow Cytometry
• Primer Design
• Gel Electrophoresis
• Immunoprecipitation
• Genotyping
• Recombinant Dna Technology
• Animal Work
• Transfection
• Sirna
• Mouse Handling
• Reporter Gene Assays
• Protein Expression
• Confocal Microscopy
• Drug Testing
• Real-Time Pcr
• Plasmid Isolation
• Transgenic Mice
• Assay Development
• Pcr
• Co-Immunoprecipitation
• Molecular Cloning
• Animal Models
• Sds-Page
• Viral Vectors
• Immunofluorescence
• Gene Expression
• Co-Ip
• Mouse Models
• Pcr Primer Design