Chandrasekar Narayanappa

About Chandrasekar Narayanappa

I am a Biotechnology postgraduate with over 5 years of combined academic and industrial experience in protein engineering, synthetic biology, and microbial strain development. Currently, I serve as a Senior Research Associate at Laurus Bio Pvt. Ltd.(formerly Richcore Lifesciences), Bangalore, where I specialize in molecular cloning, strain engineering, and clone characterization for recombinant protein production in E. coli and Pichia pastoris.My work spans the complete pipeline of recombinant protein expression—from vector construction and cloning to expression screening, media optimization, and clone selection. I have hands-on expertise in molecular biology techniques such as restriction digestion, PCR, cDNA synthesis, plasmid linearization, gel purification, transformation, SDS-PAGE, and Western blotting. I also support downstream processes, including the development of Research and Master Cell Banks (RCB/MCB), and ensure strain stability and purity using viability assays, plaque testing, and differential staining.Previously, I have contributed to the development of PCR-based diagnostics and engineered microbial strains for expressing growth factors used in cultivated meat applications. I’m particularly passionate about applying synthetic biology and systems-level approaches to build high-yield, scalable, and genetically stable microbial platforms for sustainable biomanufacturing.With a strong foundation in strain development and process optimization, I aim to grow in roles that blend molecular biology, applied biotechnology, and innovation-driven research for industrial and therapeutic applications.

Experience

1. Principal Scientist

   Biocon Biologics

   Jul 2025 — Present · Bengaluru South, IN

   Principal Scientist | Molecular Biology & Cell Line Development Expert in recombinant protein expression and microbial strain engineering, with a focus on Pichia pastoris and E. coli systems.• Designed and optimized proteins for enhanced solubility, yield, and functionality using rational engineering strategies.• Engineered Pichia pastoris strains using CRISPR-Cas9 and Cas12a (Mad7) systems for high-efficiency expression of therapeutic proteins.• Developed high-throughput cloning workflows leveraging S. cerevisiae-based homologous recombination, Golden Gate, and Gibson Assembly to accelerate construct generation.• Led expression optimization from vector design to small- and mid-scale production, collaborating closely with upstream, downstream, and analytical teams.• Proficient in PCR, SDS-PAGE, Western blotting, and sequence analysis to validate construct integrity and expression efficiency. Delivered high-yield recombinant proteins for R&D and process scale-up Shortened construct development timelines through automation and optimized cloning strategies Played a key role in cross-functional tech transfer and fermentation readiness for product development

Education

• MGR college , Hosur

  Bachelor's degree, Biotechnology

  2013 — 2016

• The Oxford College of Science, Bengaluru.

  Master's degree, Biotechnology

  2017 — 2019

• Guru Dronacharya School of Excellence, Onnalvadi

  SSLC, SSLC

  2009 — 2011

• Maharishi vidhya mandir

  Higher secondary, PCBM

  2011 — 2013