Chad Toledo
Senior Principal Scientist at Pfizer
- Role
- Senior Principal Scientist at 辉瑞
- Location
- San Diego, CA, US
- LinkedIn followers
- 500 followers
About Chad Toledo
During my graduate and post-doctoral studies, I studied Glioblastoma Multiforme (GBM), the most common and aggressive form of brain cancer. To identify new therapeutic targets for GBM, I employed functional genetic technologies, shRNA and CRISPR-Cas9, in patient-derived GBM stem-like cells (GSCs) and also primary non-transformed human neural stem cells (NSCs), non-neoplastic tissue of origin controls, to identify genes differentially require for GBM patient isolates, but which have non-essential or redundant functions in neural stem cells.The ubiquitin proteasome system is a dynamic and complex regulated network that controls the fate of cellular proteins. At Nurix, I utilized my expertise to perform target discovery and validation studies in oncology and immuno-oncology. In addition, I assessed small molecules and lead compounds that enhance or inhibit the activity of the targeted ligase.Summary of Qualifications• Ph.D. in Molecular and Cellular Biology.• Over 13 years of hands-on experience in cellular and molecular biology and 2+ years in biotechnology.• Extensive knowledge in: cancer biology, especially glioblastoma multiforme (GBM), stem cell biology, ubiquitin proteasome system and ligase biology, immune-oncology, kinetochore biology, tumor microenvironment, cell cycle, mitosis, si/shRNA, and CRISPR-Cas9.• Extremely skilled in: mammalian cell culture (including patient-derived GBM isolates, primary neural stem cells, suspension cells, among others), assay development, Meso Scale Discovery assays, viral transductions, high-throughput shRNA and CRISPR-Cas9 screening for target discovery, next generation sequencing, RNA-Seq, cell line generation, genomic engineering, viability and phenotypic assays, multi-flow cytometry, FACS, ELISAs, immunohistochemistry, and time-lapse microscopy.• Performed multiple genome-wide and focused viability screens in preclinical in vitro and in vivo GBM models for target discovery.
Experience
Senior Principal Scientist
May 2023 — Present · San Diego, CA, US
Education
University of Washington
Doctor of Philosophy (Ph.D.), Molecular and Cellular Biology
2010 — 2015
University of Minnesota
Bachelor's Degree, Genetics, Cell Biology, and Devlopment
2005 — 2009
Skills
- Cell
- Western Blotting
- In Vivo
- Microscopy
- Sequencing
- Cancer Biology
- Gel Electrophoresis
- Lentiviral and Retroviral Production
- Cell Biology
- Biology
- Data Analysis
- Transfection
- Handling Nod-Scid Mice
- Self-Renewal and Differentiation Assays
- Biotechnology
- Protein Purification
- Stem Cells
- Angiogenic Assays
- Cell Viability Assays
- Neural Stem Cells
- In Vitro
- Dna
- Drug Discovery
- Flow Cytometry
- Genetics
- Immunoprecipitation
- Tissue Culture
- Immunohistochemistry
- Qpcr
- Rnai/Crispr Screens
- Cancer Research
- Cell Based Assays
- Molecular & Cellular Biology
- Cell Culture
- Cancer
- Gene Set Enrichment Analysis
- Molecular Biology
- Fluorescence Microscopy
- Life Sciences
- Drug Inhibitor Assays
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