Bikash Shakya
Scientist, Translational Biology at Life Edit Therapeutics
- Role
- Scientist at Life Edit Therapeutics
- Location
- Raleigh-Durham-Chapel Hill, NC, US
- LinkedIn followers
- 500 followers
About Bikash Shakya
Quick and enthusiastic learner with strong time and resource management skills and productivity as demonstrated by managing multiple research projects leading to 3 research collaborations and 15+ peer-reviewed publications.Knowledgeable mentor with expertise in CRISPR/Cas9 genome editing, large scale biochemical screening and 7+ years of laboratory experience in Microbiology, Biochemistry and Cell & Molecular Biology as evidenced by the development and optimization of 4 experimental protocols and research mentoring of 8+ undergraduate and graduate students.Excellent communicator with good scientific writing skill as demonstrated by writing 2 research grants, 12+ conference presentations with 2 best presenter awards and 4+ years of undergraduate teaching experience.Let\'s connect! b••••••••@gmail.com
Experience
Scientist
May 2022 — Present · Raleigh-Durham-Chapel Hill, NC, US
Drive large-scale CRISPR screenings in various mammalian cell types (primary and immortalized) to discover and optimize lead nucleases, base editors, and guide RNAs.Build in vitro disease models by creating CRISPR knock-in cell lines, supporting the development and testing of next-gen genome editing tools.Develop and fine-tune reporter cell lines using lentiviral systems for efficient and scalable screening workflows.Design and execute functional assays to assess the performance of engineered base editors in vitro.Collaborate across multiple projects and teams, sharing insights and best practices to accelerate scientific progress and innovation.
Education
Tribhuvan University
Master of Science (MSc), Medical Microbiology
2003 — 2005
Purdue University
Doctor of Philosophy (Ph.D.) Candidate, Microbiology/ Molecular Parasitology
2011 — 2016
Stanford University School of Medicine
Molecular Parasitology
2017 — 2019
Skills
- Data Analysis
- Elisa
- Polymerase Chain Reaction (Pcr)
- Life Sciences
- Research
- Laboratory
- Event Planning
- Molecular Cloning
- Science
- Biochemistry
- Laboratory Skills
- Pcr
- Yeast Two-Hybrid
- Collaborative Problem Solving
- Event Management
- Affinity Purification
- Genetics
- Cell Biology
- Genomics
- Microscopy
- Teaching
- Statistics
- Immunology
- Molecular Biology
- Microbiology
- Plasmodium Transfection
- Grant Writing
- Project Planning
- Western Blotting
- Lifesciences
- Protein Expression
- Confocal Microscopy
- Affinity Chromatography
- Biotechnology
- Protein Purification
- Flow Cytometry
- Cell Culture
- Bioinformatics
- Microsoft Excel
- Gel Electrophoresis
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