Brian Holder

About Brian Holder

Scientist with diverse skillset in small and large molecule- Experience with Small molecules, PEGylated proteins, biotherapeutic proteins, monoclonal antibodies (mAbs), viral vectors- Assays/Test Methods:(RP-HPLC, HILIC, IEX, SEC), UV-Vis, LC-MS, CE-SDS, cIEF, AUC, Compendial• Assay method development, transfer, validation skillset. • Works/Collaborates across various CMC functions.• Analytical Platforms: UHPLC Pumps (Agilent Technologies, Shimadzu, Waters Acquity, Perkin Elmer Flexar), MS/MS (AB SCIEX QqQ, QTRAP, Perkin Elmer QSight), FPLC (AKTA Systems), ADDA, HTC Pal Autosamplers. • Data Analysis: Analyst, MultiQuant, Unicorn (AKTA Systems), LeadScape, Radmis LIMS, Empower, Chromeleon.

Experience

1. Principal Scientist

   GSK

   Sep 2021 — Present · Upper Merion, PA, US

   Preclinical Analytical Development Scientist. Practitioner in Separations Science involving many drug modalities: mAbs, antibody drug conjugate, small molecules etc.

Education

• University of the West Indies, Cave Hill Campus

  Bachelor of Science (B.Sc.), Biochemistry with Chemistry

  2001 — 2004

• University of Nottingham

  Master of Science (M.Sc.), Applied Biomolecular Technology

  2004 — 2006

• University of Connecticut

  Master of Science (M.S.), Biochemistry

  2008 — 2012

Skills

• Fluorescence Spectroscopy
• Molecular Biology
• Organizational Effectiveness
• Chromatography
• Drug Development
• Genetics
• Protein-Protein Interactions
• Pcr
• Biochemistry
• Organic Chemistry
• Elisa
• Molecular Cloning
• Mass Spectrometry
• Science
• Antibodies
• Microscopy
• Analytical Method Validation
• Gel Electrophoresis
• Spectrophotometry
• Purification
• Protein Expression
• Western Blotting
• Wet Chemistry
• Technical Documentation
• Spectroscopy
• Nanoparticles
• Lims
• Uv-Vis
• Uv/Vis
• Cell Culture
• Bioinformatics
• Innovation
• Dna Extraction
• Productivity
• Protein Chemistry
• Microbiology
• Research
• Fplc
• Sds-Page
• Analytical Chemistry