Bhargavi Narayanan
Gene therapy | Technical Operations | Program Management | Quality Assurance
- Role
- Associate Director, Quality Assurance at Ultragenyx
- Location
- Boston, MA, US
- LinkedIn followers
- 500 followers
About Bhargavi Narayanan
Protein biochemistry: Antibody/antigen purifications using FPLC AKTA system, enzyme conjugations, electrophoretic methods- SDS-PAGE, western blotting Molecular biology: DNA/RNA-isolation, purification & quantification, primer design, PCR, cloning, in vitro transcription, transfection (lipofection/electroporation), DNA sequence analysis, qPCR & RT-qPCR Virology: Purification and radiolabeling virus, characterization assays- titer assay, infection assay, binding assay, small & large scale lentiviral vector production & purification Cell culture: Adherent & suspension cell culture, use of cell factories, Producer cell line development: Lentiviral vector design and construction, stable cell line generation, screening and top clone identification, characterization and scalability studies, primary cell transduction (T cells, CD34) for VCN Bioprocess techniques: Small scale bioreactors; separation processes-scrubber, distillation unit, biofiltration and centrifugatio8n methods, tangential flow filtration Immunofluorescence staining, flow cytometry & FACS Nuclease-mediated gene editing
Experience
Associate Director, Quality Assurance
Mar 2025 — Present
Education
University of Pennsylvania
Masters, Biotechnology
Shanmugha Arts, Science, Technology and Research Academy
Bachelors
Skills
- Sds-Page
- Site-Directed Mutagenesis
- Primer Design
- Cell
- Western Blotting
- Purification
- Protein Expression
- Microbiology
- Molecular Biology
- Fluorescence Microscopy
- Life Sciences
- Cell Culture
- Chromatography
- Flow Cytometry
- Pcr
- Biochemistry
- Biotechnology
- Sequencing
- Elisa
- Laboratory
- Gel Electrophoresis
- Immunology
- Polymerase Chain Reaction (Pcr)
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