Berket Khalifa
Researcher @King Faisal Specialist Hospital and Research Center
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WORK HISTORY
Researcher @King Faisal Specialist Hospital and Research Center
Research Associate • Introduced Automation expertise for Molecular Bio Medicine Program by setting up drug screening to increase productivity, accuracy and reproducibility. • Set up and wrote multiple automated programs for gene reporter assays to screen a panel of kinase compound libraries using Hamilton liquid handling robot. • Performed multiple optimization experiments to validate automated gene reporter assay to identify an optimal condition and to study the effect of drugs on transfected cells using 384-well format. • Played a role of compound management for research center and responsible in handling requested compounds and deliver to team members in a timely manner.
EDUCATION
University of California, Irvine, CA
Bachelor of Science, Biological Sciences
SKILLS
ABOUT BERKET KHALIFA
Creative senior research scientist with extensive experience in drug discovery performing basic research and target validation studies for various research projects. Highly skilled in assay development, validation, automation, and miniaturization of biochemical and cell-based medium- to high- throughput screening assaysSpecialties: • Assay development, validation, automation,/ MTS / Drug combinations • High Content Screening (HCS). • Target Validation studies • Isolation, culturing and maintenance of human primary cells.• Cell signaling pathway analysis• Miniaturization of biochemical and cell-based assays( ELISA/ Western/ MSD-Meso/TaqMan: qPCR TLDA /FP/TR-FRET/Alpha Screen )• Data analysis: Graph P. Prism, Excel, Activity Base, SQL*LIMS, TIBCO Spot fire, Gene Data/MATLABOTHER SKILLS• Six Sigma-Yellow Belt certified• Assay development and automation for MTS and HTS screening• Implementation of ISO 9001, 1348 and 17025 guidelines.
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