Barbara Sullinger
Protein Scientist @Vaxcyte
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WORK HISTORY
Protein Scientist @Vaxcyte
US
Currently working in R&D on the development of next-generation vaccines using the XpressCF® cell-free protein synthesis platform-Designed target antigens through gene engineering, epitope mapping, and sequence optimization to ensure solubility, proper folding, and conjugation readiness-Scaled upstream expression using DasBox, Eppendorf BioFlow 120, and AMBR systems; optimized pH, DO, and temperature for high yield-Developed multi-step purification workflows (SEC, HIC, affinity, ion exchange) on AKTA FPLC to improve purity, yield, and scalability-Identified critical process parameters (CPPs) and characterized process robustness to support regulatory documentation and tech transfer-Executed analytical assays (SEC-MALS, BCA, SDS-PAGE, Western blot) and stability studies (Uncle) to confirm antigen integrity and consistency-Prepared antigens for preclinical studies, measured IgG titers via ELISA, and introduced INTEGRA automation to improve assay reproducibility.
EDUCATION
University of California, Berkeley, Haas School of Business
Master of Business Administration - MBA, Business Administration and Management, General
University of California, Berkeley
Molecular cell biology, Neuroscience
ABOUT BARBARA SULLINGER
Biotech professional with 10+ years at the intersection of vaccine R&D and strategic…
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