Aram Chang

About Aram Chang

Profile:Protein biochemist and structural biologist with 18+ years of experience, including 7+ years in the pharmaceutical industry. Expertise in antibody and protein production at various scales. Skilled in leading cross-functional teams, managing biotherapeutic discovery programs. Extensive experience in membrane protein production and in overcoming technical challenges in protein expression and purification.• Development and production of diverse antibody formats (monoclonal, bispecific, multispecific, Fc-fusions), including construct design, expression optimization, and purification. • Biotherapeutic project lead and cross-functional team management, Team leadership in protein production. • Expertise in biotherapeutics leads discovery, with a focus on developability, risk mitigation, and manufacturability assessment. • Extensive experience in membrane protein production for structural studies, including Cryo-EM and biophysical characterization.• Collaborated with computational biology teams to incorporate AI and machine learning-driven approaches into protein design and production.Technical Skills• Antibody Production & Engineering: Monoclonal, bispecific, multispecific, Fc-fusion proteins; engineering for stability and function; manufacturability assessments. • Protein Expression: Expertise in prokaryotic (E. coli) and eukaryotic (HEK cells, baculovirus, mammalian, hybridoma) systems. Expression optimization using DoE (Design of Experiments). • Protein Purification: AKTA FPLC, Pure, Avant, HPLC Agilent systems. Techniques include affinity chromatography (His-tag, Strep-tag), ion exchange (IEX), SEC, HIC, and more. • Structural Biology and modeling: X-ray crystallography (manual and robotic crystallization, synchrotron data collection, experimental phasing, molecular replacement), Cryo-EM (CryoSPARC), analysis (pymol, UCSF Chimera), and modeling (AlphaFold). • Biophysical Characterization: LC-MS, SDS-PAGE, Western Blot, SEC-MALS, Mass Photometry, nanoDSF, ITC, CD.• Biologics Data Management: Genedata

Experience

1. Principal Scientist

   Bristol Myers Squibb

   Jun 2022 — Present · Redwood City, CA, US

   Led the Protein Science Purification and Antigen Technology group, producing therapeutic antibodies (monoclonal, bispecific, multispecific) and complex protein antigens at 100 mL to 50 L scale. Standardized workflows for quality control, data management, and sample delivery. • Served as Project Lead in Biotherapeutics. Collaboration with other companies.• Led the protein production team. Manage 3 team members, including Ph.D. level scientists and contractors.• Built membrane protein production platforms supporting Cryo-EM, HDX, and mechanistic studies. • Evaluated the manufacturability assessment to support transition to CMC. • Worked closely with CRO for the protein production and structural study using cryo-EM.• Partnered with the computational biology team to support AI-ML designed protein production

Education

• University of Wisconsin-Madison

  Ph.D., Biophysics/Structural Biology

• Seoul National University

  B.S., Biological Science

Skills

• Pcr
• Molecular Cloning
• Science
• Biochemistry
• Structural Biology
• Biophysics
• Cell Culture
• X-Ray Crystallography
• Negative Staining Electron Microscopy
• Purification
• Protein Expression
• Small Angle X-Ray Scattering
• Protein Chemistry
• Molecular Biology
• Protein Purification
• Western Blotting
• Research