Alan Rosales

About Alan Rosales

I am currently a Scientist I in the Gene Therapy R&D group at Sarepta Therapeutics. I earned a PhD in Biomedical Engineering from Dr. Aravind Asokan’s Lab at Duke University, where my research was dedicated to pioneering advancements in gene therapy through the evolution of adeno-associated viruses (AAV). One focus of my work was engineering novel AAV variants capable of robust and effective gene transfer across multiple species, with a specific emphasis on kidney-targeted therapies. These efforts led to the discovery of new AAV variants with enhanced capabilities in diverse kidney models, ranging from murine to porcine, human kidney organoids, and nonhuman primate kidneys.My role extended beyond benchwork; I conceptualized, designed, and executed both in vivo and in vitro studies, contributing to a deeper understanding of AAV-mediated gene transfer. This research spanned multi-scale model systems, from organoids to large animal models, enabling translational insights that bridged the gap between laboratory findings and clinical application.

Experience

1. Scientist i, Gene Therapy R & d

   Sarepta Therapeutics

   Nov 2024 — Present · Columbus, OH, US

   Gene Therapy R&D Group:I focus on target discovery, characterization of preclinical animal models of neuromuscular and cardiac diseases, and demonstration of the mechanism of action of gene therapies through in vitro and in vivo preclinical studies. I also assist in designing and executing critical preclinical studies that evaluate efficacy and safety, helping to bridge the gap between early research and clinical application.

Education

• Duke University

  Doctor of Philosophy, Biomedical Engineering

• Medgar Evers College Preparatory School

  High School Diploma

  2006 — 2010

• University of Rochester

  Bachelor of Science, Biomedical Engineering

  2010 — 2014

• The City College of New York

  Master of Science, Biomedical Engineering

  2017 — 2019

Skills

• Statistics
• Genetics
• Immunoprecipitation
• Nucleofection
• Microsoft Word
• Animal Work
• Viral Vectors
• Transfection
• Mammalian Cell Culturing
• C++
• Protein Assays
• Data Analysis
• Imagej
• Clc Sequence Viewer
• Bacterial Culturing
• Elisa
• Matlab
• Gel Electrophoresis
• Image Analysis
• Research
• Microsoft Powerpoint
• Bsl2+ Trained
• Dna Sequencing
• Ir Spectroscopy
• Teamwork
• Pcr
• Virus Culture
• Java
• Laboratory Techniques
• Leadership
• Bacterial Transformation
• Mouse Models
• Rna Isolation
• Laboratory Animal Medicine
• Lentivirus
• Transduction
• Cell Culture
• Virology
• Transgenic Mice
• Bacterial Culture