Akash Kumar

About Akash Kumar

I am an experienced bio-pharmaceutical scientist with a decade of experience in industrial R&D. I have a diverse background in both vaccine development and drug discovery, and currently specialize in CRISPR-based technologies for gene editing and diagnostics. I manage projects with a holistic approach, paying attention to details, and believe in the power of collaboration, continuous learning, and gratitude for success in the scientific field. Accomplishments: CRISPR-Cas12 approach to develop a diagnostic assay for detection of unique DNA sequences. Immunological assays for CoVID-19 using spike and nucleocapsid of SARS-CoV2. Nanobody diversity generation and selection against SARS-CoV2 spike protein. CRISPR-Cas and homologous recombination approach to generate Brucella abortus knockouts. Disk mass optimization and breakpoint analysis for the in-house developed NCEs following the CLSI guidelines. PhD in Biotechnology (Bacterial Genetics); Published 8 international research papers Expertise: Gene deletion/knockout (CRISPR-Cas and homologous recombination approach), Molecular cloning (recombinant DNA technology) and site-directed mutagenesis, Immunological (LFT) and nucleic acid (RT-PCR) based diagnostics development, Protein expression (bacterial, yeast and mammalian system) and purification, Antimicrobial susceptibility testing, biofilm formation and eradication assays, In vitro new chemical entity screening and SAR analysis, Enzyme activity and inhibition assay development, Routinely used laboratory techniques in Microbiology, Biochemistry and Molecular biology; Instrumentation – Confocal microscopy, UV-Visible and Fluorescence spectrophotometer, Gel electrophoresis; Software – Statistical data analysis, Fluoview, Microsoft office, Openoffice; Soft skills – Aptitude to critically design and implement experiments, good oral/written communication skills, ability to promote collaboration across teams, competence to instruct and train others. DISCLAIMER: The views expressed in my LinkedIn posts are my own and not those of my employers or LinkedIn. I am not responsible for comments made by others on my posts. The content I share is for informative purposes only, and any reliance on it is at your own risk. For copyright concerns, please contact me. Names and logos mentioned are owned by their respective companies. I may update this disclaimer as I learn more.

Experience

1. Senior Manager

   Indian Immunologicals Limited

   Nov 2023 — Present · Hyderabad, IN

Education

• Gujarat Vidyapith

  Master's

• Kendriya Vidyalaya

  X & XII

• Indian Institute of Technology, Kharagpur

  PhD

• Gujarat University

  Bachelor's