Aayush K.

About Aayush K.

Over 9 years of experience in Process Development of monoclonal antibodies(recombinant proteins) and fusion proteins using mammalian cell lines.• Competent in developing process wıth AMBR 15, Applikon minibioreactor, shake flask, DasGip (2L), Sartorious bioreactor (2 to 10 L scale- B plus and B-DCU II), Repligen perfusion technology (ATF4 - 10L scale), Wave Bioreactor (50L scale) & XDR (GE healthcare 50L).• Proficient in working with glass bioreactors and single use technology in GMP Laboratory.• Carried out Process Characterization of fusion protein using DOE.• Demonstrated ability in setting up Lab (Procurement and installation of equipment).

Experience

1. Research Associate

   Lupin

   Oct 2016 — Present · Pune, IN

   Ordering and maintaining laboratory’s inventory.• Preparation of media, feeds, reagents & buffers. • Revival/Preparation of Working cell bank.• Mammalian cell banks thawing, passaging & seed train development.• Clone selection, media and feeds screening and process development using AMBR 15.• In process sampling and routine metabolites’ measurement (CEDEX Biochemical Analyzer).• Process parameter optimization in DasGip (2L) bioreactor using DOE.• Scale up studies from DasGip (2L) bioreactor to Sartorius bioreactors (5L & 10L).• Plaining and execution of experiments in 10L bioreactors using Alternating Tangential Flow (ATF4) system (Perfusion). • Seed train development & execution of experiments in 50L bioreactor (Xcellerex).• Designing of experiments using DOE software (Design Expert).• Clarification of mAbs using depth filters (Merck Millipore).• Proetin estimation using Protein A resins.• Review BPRs and technical records for completeness and accuracy of data.• Write/revise/review SOP\'s, risk assessments, protocols and reports.• Troubleshooting of Laboratory equipment. • Cross functional department co-ordination.• Monthly/Quaterly data presentation and project progress update.

Education

• Rajiv Gandhi Proudyogiki Vishwavidyalaya

  Master of Technology (M.Tech.), Biotechnology

  2011 — 2013

• Rajasthan Technical University

  Bachelor's degree (B.Tech), Biotechnology

  2006 — 2010