Aakash Kabbin

Scientist i @insitro

South San Francisco, CA, US

Adipose Organoids- Generated adipose organoids with unilocular lipid morphology using SVF-derived cells. Established flow cytometry panels to characterize SVF populations, optimizing depot-specific differentiation- Performed bulk and single-nuclei RNA-seq to confirm all major adipose cell types and enhanced metabolic pathways- Developed organoid cross-sections, immunostaining panels to validate tissue-like architecture and and a lipolysis assay for functionality.Adipocyte Modeling – using ASC52telo- Engineered MSCs with adipocyte-specific transcription factors to achieve up to 99% differentiation into white, beige, and brown adipocytes- Established single-nuclei RNA-seq workflows, increasing nuclei yield by 10x. Applied siRNA and CRISPR-KO to induce thermogenically active beige adipocytes and assess transcriptional and functional changes- Developed a beige adipocyte ICC panel and partnered with imaging teams to integrate cells into high-throughput, imaging-based screening platforms.Hepatocyte Modeling – using iPSCs- Engineered iPSCs with hepatocyte-specific transcription factors and a custom small-molecule cocktail to increase mature hepatocyte yield to 75%- Converted 3D iHEP cultures into imaging-compatible monolayers- Conducted pooled optical CRISPR screening (POSH) for MASLD target discovery and established lipid accumulation assays with ML-driven phenotypic analysis- Evaluated Quantitative Phase Imaging (QPI) on 3D hepatocyte spheroids to identify cellular organelles and morphological features.

University of Southern California

Master of Science (M.S.), Stem Cell Biology and Regenerative Medicine

Northeastern University

Master of Science (M.S.), Biotechnology

D.Y. Patil University, School of Biotechnology and Bioinformatics

Bachelor of Technology (B.Tech.), Biotechnology